Lab test · Immunology

25OH Vitamin D,D2,D3 Ultrasensitive by LCMS

This test measures the levels of Vitamin D2 and D3 in the blood using advanced LCMS technology.
4.912,480+ patients have rated Prima
NABL-certified lab Free home collection Report in 24 hrs
ReportWithin 24 hrs
SampleBlood
CollectionHome or centre
FastingNot needed
How it works
1
Book in a minute
Add to cart, pick a date and time slot, pay online or at collection.
2
Sample collected at home or centre
A trained phlebotomist arrives in your slot with sealed, single-use kits.
3
Report on WhatsApp
Within 24 hours on WhatsApp and under My Orders.
Preparation
No special preparation needed unless your doctor advises otherwise.
About this test

This test measures the levels of Vitamin D2 and D3 in the blood using advanced LCMS technology. It helps assess vitamin D deficiency and overall bone and immune health.

25OH VITAMIN D,D2,D3 ULTRASENSITIVE BY LCMS The test is a highly accurate blood test that measures the total level of Vitamin D in the body, including both Vitamin D2 (ergocalciferol) and Vitamin D3 (cholecalciferol). This test uses LCMS (Liquid Chromatography-Mass Spectrometry), a highly sensitive and precise method for detecting even small amounts of vitamin D. This test is the gold standard for accurately measuring and differentiating 25-hydroxyvitamin D2 and D3 levels in the blood.

The 25-OH Vitamin D Test is performed to:

  • Measure total Vitamin D levels (D2 and D3)

  • Detect vitamin D deficiency or insufficiency

  • Assess bone health and calcium metabolism

  • Monitor vitamin D supplementation therapy

25OH VITAMIN D,D2,D3 ULTRASENSITIVE BY LCMS Test Purpose

The 25OH Vitamin D, D2, D3 Ultrasensitive by LCMS Test is performed to accurately measure the levels of 25-hydroxyvitamin D2 and D3 in the blood using Liquid Chromatography–Mass Spectrometry (LCMS), a highly sensitive and precise testing method. Primarily, the test is used to assess vitamin D status and detect deficiency or insufficiency. Additionally, it helps evaluate bone health, calcium metabolism, and overall nutritional status. Furthermore, the test assists healthcare providers in diagnosing conditions related to vitamin D deficiency, monitoring vitamin D supplementation, and assessing patients with disorders affecting vitamin D absorption or metabolism. Moreover, the LCMS method distinguishes between vitamin D2 and D3, providing a more comprehensive evaluation when compared with routine vitamin D testing.


25OH VITAMIN D,D2,D3 ULTRASENSITIVE BY LCMS Test is Recommended for Individuals:

  • Individuals with suspected vitamin D deficiency or insufficiency.
  • People experiencing bone pain, muscle weakness, or frequent fractures.
  • Individuals diagnosed with osteoporosis or osteomalacia.
  • Patients receiving vitamin D supplementation who require treatment monitoring.
  • Individuals with kidney, liver, or gastrointestinal disorders that affect vitamin D absorption or metabolism.
  • Older adults are at increased risk of low bone density.
  • Women who are pregnant or breastfeeding, when advised by their healthcare provider.
  • Individuals with limited sun exposure or dietary vitamin D deficiency.
  • Patients with disorders affecting calcium or phosphate metabolism.

FAQs

1. What is the 25OH Vitamin D, D2, D3 Ultrasensitive by LCMS Test used for?
The test measures 25-hydroxyvitamin D2 and D3 levels in the blood using the highly accurate LCMS method. Additionally, it helps detect vitamin D deficiency, assess bone health, and monitor vitamin D therapy.

2. Is fasting required before the 25OH Vitamin D, D2, D3 Ultrasensitive by LCMS Test?
No. Fasting is generally not required for this test. However, any specific preparation instructions provided by your healthcare provider should be followed.

3. Why is the LCMS method used for this test?
The LCMS (Liquid Chromatography–Mass Spectrometry) method provides a highly accurate and sensitive measurement of vitamin D levels. Furthermore, it distinguishes between vitamin D2 and vitamin D3, making it especially useful for monitoring supplementation and evaluating complex vitamin D disorders.

What patients say about Prima
4.9
12,480+ patients rated Prima Diagnostics
RK
Ramesh K.
Verified · 2 weeks ago
5.0
Quick appointment and the staff explained everything. Report came the same evening on WhatsApp.
Booked: MRI Brain
SP
Sneha P.
Verified · 1 month ago
4.5
Booking on the app was simple and pricing was clear with no hidden charges.
Booked: Full Body Checkup
AM
Anita M.
Verified · 1 week ago
5.0
Home collection was right on time and completely painless. Very professional.
Booked: Thyroid Profile
VS
Vikram S.
Verified · 3 days ago
5.0
Great value and fast report. Booked at 9am and had results by evening.
Booked: CBC
Questions people ask
How soon will I get my report?
Your report is delivered within 24 hours of sample collection — on WhatsApp and under My Orders.
Is home sample collection available?
Yes. A trained phlebotomist visits your home with sealed, single-use kits. Home collection is free.
Do I need to fast or prepare?
No fasting is required for this test unless your doctor advises otherwise.
How do I pay?
Pay online (UPI / cards) or pay at collection or at the centre. The price you see is what you pay — it includes the report, with no hidden charges.
Is the lab accredited?
Yes. Prima Diagnostics runs NABL-certified labs with strict quality control across 7 centres in Bengaluru.